| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
18/02/2014 |
|
Data da última atualização: |
05/02/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
ARASHIRO, E. K. N.; PALHÃO, M. P.; WOHLRES-VIANA, S.; SIQUEIRA, L. G. B.; CAMARGO, L. S. de A.; HENRY, M. R. J. M.; VIANA, J. H. M. |
|
Afiliação: |
EDUARDO KENJI N. ARASHIRO, UFMG; MILLER PEREIRA PALHÃO, UNIFENAS; SABINE WOHLRES-VIANA, UFJF; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MARC ROGER JEAN MARIE HENRY, UFMG; JOAO HENRIQUE MOREIRA VIANA, CNPGL. |
|
Título: |
In vivo collection of follicular fluid and granulosa cells from individual follicles of different diameters in cattle by an adapted ovum pick-up system. |
|
Ano de publicação: |
2013 |
|
Fonte/Imprenta: |
Reproductive Biology and Endocrinology, v. 11, article 73, 2013. |
|
DOI: |
https://doi.org/10.1186/1477-7827-11-73 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Background: Most studies on granulosa cell (GC) function in cattle have been performed using GC and follicular fluid (FF) samples collected from slaughterhouse ovaries. Using this approach, the follicular developmental stage and functional status are unknown and indirectly inferred, limiting data interpretation. Ultrasound-guided follicle aspiration has previously been used to recover GC or FF samples, but this was mostly carried out in large follicles or pools of small follicles, without recording the efficiency of recovery. The present study was aimed at adapting and evaluating an ovum pick-up (OPU) system for the in vivo recovery of FF and GC from individual follicles of different diameters. Methods: In the first trial, the losses of fluid inside the tubing system were calculated using a conventional or an adapted-OPU system. Blood plasma volumes equivalent to the amount of FF in follicles of different diameters were aspirated using a conventional OPU Teflon circuit. The OPU system was then adapted by connecting 0.25 mL straws to the circuit. A second trial evaluated the efficiency of FF recovery in vivo. Follicles ranging from 4.0 to 16.8 mm in diameter were aspirated individually using the conventional or adapted-OPU systems. A third trial assessed the in vivo recovery of GC and the subsequent amount of RNA obtained from the follicles of different diameters from Holstein and Gir cattle. Results: In Trial I, the plasma recovery efficiency was similar (P > 0.05) for the volumes expected for 12 and 10 mm follicles, but decreased (P < 0.05) for smaller follicles (45.7+/-4.0%, 12.4+/-4.3% and 0.0+/-0.0% for 8, 6, and 4 mm follicles, respectively). Using the adaptation, the losses intrinsic to the aspiration system were similar for all follicle diameters. In Trial II, the expected and recovered volumes of FF were correlated (r = 0.89) and the efficiency of recovery was similar among follicles <12 mm, while larger follicles had a progressive increase in FF losses that was not related to the tubing system. In Trial III, the number of GC and amount of RNA obtained were not affected (P > 0.05) by follicle size, but differed according to breed (615,054+/-58,122 vs 458,095+/-36,407 for Holstein and Gir, respectively; P < 0.05). Conclusions: The adapted-OPU system can be successfully used for the in vivo collection of FF and GC from follicles of different diameters. This will enable further endocrine, cellular, and gene expression analyses. MenosBackground: Most studies on granulosa cell (GC) function in cattle have been performed using GC and follicular fluid (FF) samples collected from slaughterhouse ovaries. Using this approach, the follicular developmental stage and functional status are unknown and indirectly inferred, limiting data interpretation. Ultrasound-guided follicle aspiration has previously been used to recover GC or FF samples, but this was mostly carried out in large follicles or pools of small follicles, without recording the efficiency of recovery. The present study was aimed at adapting and evaluating an ovum pick-up (OPU) system for the in vivo recovery of FF and GC from individual follicles of different diameters. Methods: In the first trial, the losses of fluid inside the tubing system were calculated using a conventional or an adapted-OPU system. Blood plasma volumes equivalent to the amount of FF in follicles of different diameters were aspirated using a conventional OPU Teflon circuit. The OPU system was then adapted by connecting 0.25 mL straws to the circuit. A second trial evaluated the efficiency of FF recovery in vivo. Follicles ranging from 4.0 to 16.8 mm in diameter were aspirated individually using the conventional or adapted-OPU systems. A third trial assessed the in vivo recovery of GC and the subsequent amount of RNA obtained from the follicles of different diameters from Holstein and Gir cattle. Results: In Trial I, the plasma recovery efficiency was similar (P > 0.05) for the v... Mostrar Tudo |
|
Palavras-Chave: |
Bovine; Follicle aspiration; Follicle biopsy. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 03310naa a2200241 a 4500
001 1980418
005 2024-02-05
008 2013 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186/1477-7827-11-73$2DOI
100 1 $aARASHIRO, E. K. N.
245 $aIn vivo collection of follicular fluid and granulosa cells from individual follicles of different diameters in cattle by an adapted ovum pick-up system.$h[electronic resource]
260 $c2013
520 $aBackground: Most studies on granulosa cell (GC) function in cattle have been performed using GC and follicular fluid (FF) samples collected from slaughterhouse ovaries. Using this approach, the follicular developmental stage and functional status are unknown and indirectly inferred, limiting data interpretation. Ultrasound-guided follicle aspiration has previously been used to recover GC or FF samples, but this was mostly carried out in large follicles or pools of small follicles, without recording the efficiency of recovery. The present study was aimed at adapting and evaluating an ovum pick-up (OPU) system for the in vivo recovery of FF and GC from individual follicles of different diameters. Methods: In the first trial, the losses of fluid inside the tubing system were calculated using a conventional or an adapted-OPU system. Blood plasma volumes equivalent to the amount of FF in follicles of different diameters were aspirated using a conventional OPU Teflon circuit. The OPU system was then adapted by connecting 0.25 mL straws to the circuit. A second trial evaluated the efficiency of FF recovery in vivo. Follicles ranging from 4.0 to 16.8 mm in diameter were aspirated individually using the conventional or adapted-OPU systems. A third trial assessed the in vivo recovery of GC and the subsequent amount of RNA obtained from the follicles of different diameters from Holstein and Gir cattle. Results: In Trial I, the plasma recovery efficiency was similar (P > 0.05) for the volumes expected for 12 and 10 mm follicles, but decreased (P < 0.05) for smaller follicles (45.7+/-4.0%, 12.4+/-4.3% and 0.0+/-0.0% for 8, 6, and 4 mm follicles, respectively). Using the adaptation, the losses intrinsic to the aspiration system were similar for all follicle diameters. In Trial II, the expected and recovered volumes of FF were correlated (r = 0.89) and the efficiency of recovery was similar among follicles <12 mm, while larger follicles had a progressive increase in FF losses that was not related to the tubing system. In Trial III, the number of GC and amount of RNA obtained were not affected (P > 0.05) by follicle size, but differed according to breed (615,054+/-58,122 vs 458,095+/-36,407 for Holstein and Gir, respectively; P < 0.05). Conclusions: The adapted-OPU system can be successfully used for the in vivo collection of FF and GC from follicles of different diameters. This will enable further endocrine, cellular, and gene expression analyses.
653 $aBovine
653 $aFollicle aspiration
653 $aFollicle biopsy
700 1 $aPALHÃO, M. P.
700 1 $aWOHLRES-VIANA, S.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aCAMARGO, L. S. de A.
700 1 $aHENRY, M. R. J. M.
700 1 $aVIANA, J. H. M.
773 $tReproductive Biology and Endocrinology$gv. 11, article 73, 2013.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 244 | |
| 121. |  | CASTRO, L. A. S. de; THÜRMER, L. Potencial produtivo de cinquenta acessos genéticos de batata-doce do banco ativo de germoplasma da Embrapa Clima Temperado na safra 2009/2010. In: SIMPÓSIO ESTADUAL DE AGROENERGIA, 3.; REUNIÃO TÉCNICA ANUAL DE AGROENERGIA, 3.; REUNIÃO TÉCNICA DA MANDIOCA, 10.; REUNIÃO TÉCNICA DA BATATA-DOCE, 2., Pelotas, 2010. Anais... Pelotas: Embrapa Clima Temperado, 2010. Organizado por Sérgio Delmar dos Anjos e Silva, Ivan Rodrigues de Almeida e Ana Cláudia Barneche de Oliveira. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
|   |
| 124. | | CASTRO, L. A. S. de; TREPTOW, R. de O. Caracteristicas exigidas pelos consumidores de batata-doce no Rio Grande do Sul. In: SIMPÓSIO ESTADUAL DE AGROENERGIA, 3.; REUNIÃO TÉCNICA ANUAL DE AGROENERGIA, 3.; REUNIÃO TÉCNICA DA MANDIOCA, 10.; REUNIÃO TÉCNICA DA BATATA-DOCE, 2., Pelotas, 2010. Anais... Pelotas: Embrapa Clima Temperado, 2010. Organizado por Sérgio Delmar dos Anjos e Silva, Ivan Rodrigues de Almeida e Ana Cláudia Barneche de Oliveira. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
| |
| 125. | | CASTRO, L. A. S. de; TREPTOW, R. de O. Caracterização de seis acessos de batata-doce do banco ativo de germoplasma da embrapa clima temperado em relação à cor e composição química. In: SIMPÓSIO ESTADUAL DE AGROENERGIA, 3.; REUNIÃO TÉCNICA ANUAL DE AGROENERGIA, 3.; REUNIÃO TÉCNICA DA MANDIOCA, 10.; REUNIÃO TÉCNICA DA BATATA-DOCE, 2., Pelotas, 2010. Anais... Pelotas: Embrapa Clima Temperado, 2010. Organizado por Sérgio Delmar dos Anjos e Silva, Ivan Rodrigues de Almeida e Ana Cláudia Barneche de Oliveira. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
| |
| 126. | | CASTRO, L. A. S. de; TREPTOW, R. de O. Recuperação de um genótipo de batata-doce com casca e polpa roxas. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais... Salvador: Sociedade Brasileira de Recursos Genéticos, 2010. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
| |
| 132. | | CHAVARRIA, G.; SANTOS, H. P. dos; CASTRO, L. A. S. de; MARODIN, A. B.; BERGAMASCHI, H. Anatomia, teor de clorofila e potencial fotossintético de folhas de videiras (Vitis vinifera L.) sob cobertura plástica. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20., 2008, Vitória, ES. Anais... Vitória: Incaper, 2008. Não paginado. 1 DVD.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Uva e Vinho. |
|  |
| 133. | | CHAVARRIA, G.; SANTOS, H. P. dos; CASTRO, L. A. S. de; MARODIN, G. A. B.; BERGAMASCHI, H. Anatomy, chlorophyll content and photosynthetic potential in grapevine leaves under plastic cover. Revista Brasileira de Fruticultura, Jaboticabal, v. 34, n. 3, p. 661-668, set. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 134. | | CHAVARRIA, G.; SANTOS, H. P. dos; CASTRO, L. A. S. de; MARODIN, G. A. B.; BERGAMASCHI, H. Anatomy, chlorophyll content and photosynthetic potential in grapevine leaves under plastic cover. Revista Brasileira de Fruticultura, Jaboticabal, v. 34, n. 3, p. 661-668, set. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Clima Temperado. |
| |
| 135. | | CHAVARRIA, G.; SANTOS, H. P. dos; CASTRO, L. A. S. de; MARODIN, G. A. B.; BERGAMASCHI, H. Efeito da cobertura plástica sobre anatomia, teor de clorofila e potencial fotossintético de folhas de videiras (Vitis vinifera L.) cv. Moscato Giallo. In: CONGRESSO BRASILEIRO DE VITICULTURA E ENOLOGIA, 12., 2008, Bento Gonçalves. Anais... Bento Gonçalves: Embrapa Uva e Vinho, 2008. p. 109. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 138. | | CROCHEMORE, A. G.; MATTOS, M. L. T.; VENDRUSCOLO, C. T.; CASTRO, L. A. S. de. Bactérias degradadoras do inseticida carbofurano acumuladoras de biopolímero. In: SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasilia, DF. Anais...Brasilia, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. p. 563.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
| |
| 140. | | CROCHEMORE, A. G.; MATTOS, M. L. T.; VENDRUSCOLO, C. T.; CASTRO, L. A. S. de. Microscopia eletrônica de transmissão para detecção de biopolímero em espécies de Pseudomonas. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA E PÓS GRADUAÇÃO DA EMBRAPA CLIMA TEMPERADO, 3., 2010, Pelotas. resumos e palestras... Pelotas: Embrapa Clima Temperado, 2010. Anais: Carreira, ética e inovação: o que você está fazendo? Pelotas: Embrapa Clima Temperado, 2010. Editado por Ivan Rodrigues de Almeida, Leonardo Ferreira Dutra e Jamir Luis Silva da Silva. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
| |
| Registros recuperados : 244 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
